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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Macrophage-derived exosomal microRNA-501-3p promotes progression of pancreatic ductal adenocarcinoma through the TGFBR3-mediated TGF-β signaling pathway

Fig. 6

MiR-501-3p promotes migration, invasion and tube formation of PDAC cells by down-regulating TGFBR3. a the predicted binding sites of miR-501-3p and TGFBR3 on TargetScan database. b the binding of miR-501-3p to TGFBR3 was confirmed by dual luciferase reporter gene assay. * p < 0.05 vs. mimic-NC. C. RT-qPCR detection of TGFBR3 mRNA levels in PANC-1 and BxPC-3 cells treated with miR-501-3p mimic. * p < 0.05 vs. NC mimic. d and e Western blot analysis of TGFBR3 protein in PANC-1 and BxPC-3 cells treated with miR-501-3p mimic. * p < 0.05 vs. NC mimic. f-i migration and invasion abilities of PANC-1 and BxPC-3 cells treated with miR-501-3p mimic vector, miR-501-3p mimic + oe-TGFBR3, Mp-Exo + vector, PBS + oe-TGFBR3 and Mp-Exo + oe-TGFBR3 were measured by Transwell assay (200 ×). * p < 0.05 vs. NC mimic + vector, PBS + vector. # p < 0.05 vs. NC mimic + oe-TGFBR3, PBS + oe-TGFBR3. j-m In vitro tube formation assay was used to detect HMEC-1 cell tube formation ability and quantitative analysis in response to miR-501-3p mimic vector, miR-501-3p mimic + oe-TGFBR3, Mp-Exo + vector, PBS + oe-TGFBR3 and Mp-Exo + oe-TGFBR3 (Scale bar = 100 μM). * p < 0.05 vs. NC mimic + vector, PBS + vector. # p < 0.05 vs. NC mimic + oe-TGFBR3, PBS + oe-TGFBR3. n-q Western blot analysis was used to detect migration, invasion and angiogenesis-related proteins in response to miR-501-3p mimic vector, miR-501-3p mimic + oe-TGFBR3, Mp-Exo + vector, PBS + oe-TGFBR3 and Mp-Exo + oe-TGFBR3. * p < 0.05 vs. NC mimic + vector, # p < 0.05 vs. NC mimic + oe-TGFBR3, PBS + oe-TGFBR3. R. RT-qPCR detection for mRNA expression of TGFBR3 in metastatic and non-metastatic PDAC tissues (n = 14–42). * p < 0.05 vs. non-metastatic tissue. S and T. Western blot analysis was used to detect TGFBR3 protein in metastatic and non-metastatic PDAC tissues (n = 14–42). * p < 0.05 vs. tissues without metastatic. The measurement data were expressed as mean ± standard deviation. The comparison between two groups was performed by independent sample t test. One-way ANOVA was employed for comparison among multiple groups, followed by Tukey’s post-hoc test. Cell experiments were repeated three times

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