Table 2 Primer sequences for RT-qPCR
Gene | Sequence (5'-3') | |
|---|---|---|
CD206 | F: CAAGGAAGGTTGGCATTTGT | R: CCTTTCAGTCCTTTGCAAGC |
CD68 | F: GCTACATGGCGGTGGAGTACAA | R: ATGATGAGAGGCAGCAAGATGG |
iNOS | F: ACAGGAGGGGTTAAAGCTGC | R:TTGTCTCCAAGGGACCAGG |
Arginase | F: TTGGCAATTGGAAGCATCTCTGGC | R: TCCACTTGTGGTTGTCAGTGGAGT |
GAPDH | F: ATGGAGAAGGCTGGGGCTC | R: AAGTTGTCATGGATGACCTTG |
TGFBR3 | F: CTGAAATCGTGGTGTTTAATTG | R: GCTCC ATGTTGAAGGTGATG |
CD133 | F: ACCAGGTAAGAACCCGGATCAA | R: CAAGAATTCCGCCTCCTAGCACT |
NANOG | F: TGCCTCACACGGAGACTGTC | R: TGCTATTCTTCGGCCAGTTG |
OCT4 | F: CTGAAGCAGAAGAGGATCAC | R: GACCACATCCTTCTCGAGCC |
Cyclin A2 | F: TTATTGCTGGAGCTGCCTTT | R: ACTGTTGTGCATGCTGTGGT |
Cyclin D1 | F: ACCTGGATGCTGGAGGTCT | R: GCTCCATTTGCAGCAGCTC |
Cyclin D2 | F: TTTGCCATGTACCCACCGTC | R: AGGGCATCACAAGTGAGCG |
Cyclin E1 | F: GAAGAGGAAGGCAAACGTGA | R:TGCACGTTGAGTTTGGGTAA |
p16 | F: CATAGATGCCGCGGAAGGT | R:GGATTAGGGCTTCCTCTTGGA |
p19 | F: GTC CCA GCC AGC CAT GGCAG | R: GGC CTT GCT GGG CCA TGG AG |
p21 | F: AGTCAGTTCCTTGTGGAGCC | R: CATGGGTTCTGACGGACAT |
p53 | F: GAGCCCCCTCTGAGTCAG | R: GCAAAACATCTTGTTGAG |
miR-501-3p | F: GCGGCGGAATGCACCCGGGCAAG | R: GTGCAGGGTCCGAGGT |
U6 | F: GCTTCGGCAGCACATATACTAAAAT | R: CGCTTCACGAATTTGCGTGTCAT |