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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Long non-coding RNA LUCAT1/miR-5582-3p/TCF7L2 axis regulates breast cancer stemness via Wnt/β-catenin pathway

Fig. 3

LUCAT1 enhances stem-like properties of BCCs. a Expression of stemness markers (SOX2, Nanog, CD44 and OCT4) was detected by qRT-PCR in stable LUCAT1-cDNA transfected MCF-7 and T47D cells. b Expression of stemness markers (OCT4, Nanog and SOX2) was detected in LUCAT1-overexpressing MCF-7 and T47D cells by WB. c Rate of the CD44 + CD24- cells was detected in stable LUCAT1-cDNA transfected MCF-7 and T47D cells by flow cytometry. d Proliferation ability was analyzed in LUCAT1-overexpressing MCF-7 and T47D cells by colony formation assay. Data are presented as the mean ± SD of three independent experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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