Fig. 3

Tac2-N accelerates in vivo metastasis of lung cancer. a Lungs were removed from mice injected with H1975 cells stably expressing vector control or Tac2-N and fixed with Bouin’s solution. The photograph of lungs were taken. b Lungs were removed from mice injected with H1299 cells stably expressing negative control or Tac2-N shRNA. The fluorescent images of lung tissues from nude mice were photographed. c, d IHC evaluation of vimentin in lung necropsies and quantification of metastatic deposits. Scale bar represent 100 μm. e, f Lung invaded by tumor cells were further confirmed by H&E staining. Scale bar represent 100 μm