Fig. 1

Spontaneous blebs formation and increased EVs shedding in DU145R80 compared to DU145 cells. a Fluorescent microscopy of DU145 and DU145R80 cells obtained after by FITC-CTxB membrane labelling and imaged at 40× magnification. b-c Flow cytometric detection of large EVs spontaneously shed by either DU145 or DU145R80 and collected by low speed centrifugation from conditioned serum free media. d Large EVs shedding by untreated or EGF-stimulated DU145R80 cells. e Scheme of iodixanol purified fractions of large EVs from both cell lines. f, g Tunable resistive pulse sensing analysis (by qNano Instrument) of LO from DU145 (red) and DU145R80 (blue) using NP2000 membrane pores. h LO from DU145R80: representative images by optical microscopy (50× and 100× without or with zoom magnifications)