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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: A positive feedback between IDO1 metabolite and COL12A1 via MAPK pathway to promote gastric cancer metastasis

Fig. 3

IDO1 regulates collagen genes in GC cell. a Correlation coefficient matrix diagram showed the correlation coefficients among IDO1 and collagen family genes included in WGCNA analysis. The genes were simultaneously clustered by hierarchical clustering order. The larger the dots, the stronger the correlation. Blank represented the P values ≥0.05, and other P values were less than 0.05. Effect of IDO1 down-regulation (b) or up-regulation (c) on expression of COL12A1, LOXL2, COL6A1, COL6A2, COL5A1, COL8A1 and COL13A1 in AGS and SGC-7901 or HGC-27 and MGC-803 respectively. GC cells were transfected by IDO1 siRNA or IDO1-overexpressing eukaryotic plasmid. Three days later, COL12A1, LOXL2, COL6A1, COL6A2, COL5A1, COL8A1 and COL13A1 RNA levels were detected by qRT-PCR. d Western blot was applied to examine IDO1, COL12A1, LOXL2, COL6A1 and COL6A2 protein levels in IDO1 shRNA stably expressing SGC-7901 and IDO1-overexpressing HGC-27 cell lines

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