Fig. 5

IDO1 and COL12A1 are positively associated with MAPK pathway activation in GC cells. a and b GO analysis was performed using genes with |log2 (fold change)| > 1 by comparing SGC-7901_NC with SGC-7901_shIDO1, or by comparing HGC-27_IDO1 with HGC-27_NC. Five most involved GO terms were displayed. MF: molecular function, molecular activities of gene products. CC: cellular component, gene products are active. BP: biological process, pathways and larger processes made up of the activities of multiple gene products. c Western blot was used to examine p-STAT3, p-AKT, pERK, nucleus and cytoplasm p65 after stable knockdown of IDO1 or/and COL12A1 in SGC-7901. d By establishing NC, IDO1, shCOL12A1 and IDO1 + shCOL12A1 stable HGC-27 cell lines, Western blot was used to detect p-STAT3, p-AKT, p-ERK, nucleus and cytoplasm p65. “C” and “N” represented cytoplasm and nucleus protein respectively