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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: A positive feedback between IDO1 metabolite and COL12A1 via MAPK pathway to promote gastric cancer metastasis

Fig. 6

A reciprocal positive regulation between IDO1 and COL12A1 via MAPK to promote GC cell migration. Western blot (a) and qRT-PCR (b) were used to examine IDO1 and COL12A1 expression in stable knockdown of IDO1 or/and COL12A1 SGC-7901 cell lines, or in HGC-27_NC, HGC-27_IDO1, HGC-27_shCOL12A1 and HGC-27_IDO1 + shCOL12A1 cell lines. c Transwell assay was performed to assess the migratory ability of SGC-7901_NC, SGC-7901_shIDO1, SGC-7901_shCOL12A1 and SGC-7901_shIDO1 + shCOL12A1. Similar assay was performed in HGC-27_NC, HGC-27_shCOL12A1, HGC-27_IDO1 and HGC-27_IDO1_shCOL12A1. 200×. d Western blot was utilized to detect IDO1 and COL12A1 protein levels after SGC-7901 or HGC-27_IDO1 cells treated with U0126 for 48 h. “*” represented comparing with “NC” group. **P < 0.01, ***P < 0.001. “#” represented comparing with “shIDO1 + shCOL12A1” or “IDO1” group. ##P < 0.01, ###P < 0.001

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