Fig. 6From: A positive feedback between IDO1 metabolite and COL12A1 via MAPK pathway to promote gastric cancer metastasisA reciprocal positive regulation between IDO1 and COL12A1 via MAPK to promote GC cell migration. Western blot (a) and qRT-PCR (b) were used to examine IDO1 and COL12A1 expression in stable knockdown of IDO1 or/and COL12A1 SGC-7901 cell lines, or in HGC-27_NC, HGC-27_IDO1, HGC-27_shCOL12A1 and HGC-27_IDO1 + shCOL12A1 cell lines. c Transwell assay was performed to assess the migratory ability of SGC-7901_NC, SGC-7901_shIDO1, SGC-7901_shCOL12A1 and SGC-7901_shIDO1 + shCOL12A1. Similar assay was performed in HGC-27_NC, HGC-27_shCOL12A1, HGC-27_IDO1 and HGC-27_IDO1_shCOL12A1. 200×. d Western blot was utilized to detect IDO1 and COL12A1 protein levels after SGC-7901 or HGC-27_IDO1 cells treated with U0126 for 48 h. “*” represented comparing with “NC” group. **P < 0.01, ***P < 0.001. “#” represented comparing with “shIDO1 + shCOL12A1” or “IDO1” group. ##P < 0.01, ###P < 0.001Back to article page