Fig. 2

Notch1 and CXCR4 are co-expressed in GBM tissues and enriched in GICs. a Co-immunofluorescent staining of GIC marker Nestin (in green) with CD31, Notch1, Hes1 and CXCR4 (in red) in human GBMs. Co-immunofluorescent staining of Notch1 (in green) and CXCR4 (in red) in human GBMs. b Expression heatmap of interest genes in GICs and Neurospheres relative to conventional cell lines from GEO profile (NCBI GEO datasets: GSE23806). c Pearson analysis showing a positive correlation of GIC markers with Notch1 and CXCR4 respectively from GSE23806 dataset. d Circos plots demonstrate the results of Pearson analysis