Fig. 3

Notch1 and CXCR4 presented discrepant expression patterns in GICs. a Co-immunofluorescent staining of CD133 and Nestin was performed to assess GIC phenotype, CD133 and Notch1 staining was used to identify Notch1 signaling activity in GIC tumorspheres. b Co-immunofluorescent staining of Notch1 and CXCR4 in GIC tumorspheres. c Co-immunofluorescent staining of Notch1 and F-actin d CXCR4 and F-actin. Images were captured by laser confocal microscope