Fig. 6

Notch1 enhance the activity of AKT/mTOR pathway through CXCL12 /CXCR4 axis. a and c The representative blot and relative phosphorylation of AKT and mTOR in U87GICs were shown. b and d The representative blot and relative phosphorylation of AKT and mTOR in U251GICs were shown. (*P < 0.05, **P < 0.01, ***P < 0.001, ns: no significance) e Schematic representation of the potential binding sites for RBPJ in CXCR4 promoter. and mutant type of luciferase reporter constructs have intact and mutated seed sequences. g and h Dual-reporter luciferase assays showed the relative luciferase activity of WT and Mut reporter constructs as well as Notch1 inhibition (MK0752) group. f Proposed model illustrates the underlying crosstalk between Notch1 and CXCR4 in GICs through PI3k/AKT/mTOR pathway. Notch1 signaling could interact with CXCL12/CXCR4 Chemotaxis system by directly regulating CXCR4’s promoter