Fig. 1

Identification of sgRNA candidates that confer resistance to Palbociclib. a Schematic overview of functional screening for candidate genes that confer resistance to CDK4/6 inhibition and translational workflow. b Clustering and Pearson correlation of NGS results of all biological replicates. “SAM library” indicates the amplified SAM library while 1, 2, 3 indicate independent bio-replicates of control and treatment with Palbociclib. c Individual gRNA counts were plotted for control and Palbociclib treatment conditions. Red dots indicate counts that are significantly enriched in Palbociclib treatment according to MAGeCK analysis, after applying cutoffs of p < 0.1 (FDR corrected) and LFC > 0.25