Fig. 3

Knockdown of PLCε suppresses Twist1 mRNA and protein expression in PCa cell lines. a Serum Twist1 mRNA expression in BPH, metastatic (M) and non-metastatic (NM) PCa. b Western blotting analysis detected the expression of PLCε and Twist1 in four human PCa cell lines. c, d The mRNA expression of both PLCε and Twist1 after knockdown of PLCε or Twist1 in PC3 cells line. e, f The PC3 cells were infected with three lentiviral sh-PLCε, western blotting and protein quantification analyses were detected the expression of PLCε and Twist1. g, h The PC3 cells were transfected with three sh-Twist1plasmids, western blotting analysis of PLCε and Twist1. Proteins were quantified using image software. Data were represented as mean ± SD. of three individual experiments. *p < 0.05, **p < 0.01, and *** p < 0.001 vs. Controls