Fig. 4

PLCε expression negatively correlates with PGC-1α-mediated mitochondrial oxidative metabolism and uncoupling through Twist1 in PC3 cells. a, e and i Western blotting analysis detected the expression of PLCε, Twist1, PGC-1α, CPT1B, ERRα, UCP-1, and ACADM after infected with lentiviral sh-PLCε or transfected with DDK-Twist1 or sh-Twist1 plasmids in PC3 cells. b, f and j Proteins were quantified using image software normalized against β-actin. c, g and k Seahorse tracing of the oxygen consumption rate in PC3 cells, followed by mitochondrial stress test as described in methods. d, h and l Bar graphs of means ± SD of the basal and maximal respiration and ATP production in PC3 cells. Data were represented as mean ± SD. of three individual experiments. *p < 0.05, **p < 0.01, and *** p < 0.001 vs. Controls