Fig. 8

PLCε depletion enhanced the inhibitory effect of Twist1 knockdown on PCa tumorigenesis in vivo. a The photographs of dissected tumors from nude mice were shown. b, c The growth curves and the average weights of tumors from nude mice after injection with PC3 cells infected sh-NC, sh-PLCε and sh-Twist1. Data were represented as mean ± SD. *p < 0.05. d IHC staining of PLCε, Twist1 and PGC-1α in tumor tissues from mice. Magnification × 200. Bars = 100 μm. e A mechanism for PLCε depletion-mediated Twist1 downregulation. Schematic diagram describing the functional significance of PLCε in PCa cells. PLCε may regulate Twist1 expression through MAPK-mediated ubiquitination and PPARβ-mediated transcription