Fig. 9

Effect of Sunitinib on the levels of VEGFR2 on EAhy926 surface. EAhy926 cells were incubated with Sunitinib at indicated concentrations at 37 °C for 24 h. Cells were incubated with anti-VEGFR2 antibody followed by FITC labeled secondary antibody. a VEGFR2 numbers for a no-antibody control was generated and the geometric mean calculated. This was used to control for background fluorescence. Histograms showing the number of VEGFR2 for the untreated (0 μM Sunitinib) (b) and treated (c-f) EAhy926 cells were generated, and the geometric mean calculated. g Comparison of the geometric mean of Sunitinib treated cells with the control (0 μM Sunitinib) sample (*p<0.05, **p<0.01)