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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Soluble fibrinogen-like protein 2 promotes the growth of hepatocellular carcinoma via attenuating dendritic cell-mediated cytotoxic T cell activity

Fig. 2

Fgl2 knockout activates CD8+ T cells and DCs in the tumor microenvironment of s.c. transplanted hepatomas. a Fgl2−/− and WT BALB/c mice with s.c. transplanted hepatomas were sacrificed, and cytokines in tumor homogenates were measured by ELISA or CBA at 14-days post-implantation of BNL cells. b CD3+, c CD4+, d CD8+T cells, and f DCs in tumors and DLNs were analyzed by flow cytometry. e CD8+T cells (1 × 105) from s.c. transplanted hepatomas were isolated by magnetic beads and incubated with BNL cells (1 × 105) for 4 days before measuring BNL-cell death percentage. g To further insight the influence of diminished IL-35, BNL cells were s.c. transplanted into WT BALB/c mice which were injected with 100 μg IL-35 antibody once weekly when tumor size exceeded 100mm3. Immunocytes were measured 14 days after inoculation. Data represent the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (a two-tailed unpaired Student’s t test; b-f Mann–Whitney U test) compared with negative control

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