Fig. 3

Fgl2 knockout promotes DC-mediated T cell proliferation in s.c. transplanted hepatomas. CD4⁺CD25⁻ and CD8⁺ T cells and DCs were isolated from s.c. transplanted hepatoma tissue from Fgl2^−/− or WT BALB/c mice using magnetic beads. T cells (1 × 10⁵) were dyed with CFSE (5 μM) and mixed with DCs at different ratios (DC:T cells, 1:2, 1:5, 1:10, and 1:50) in the presence of 200 IU/mL murine IL-2 in culture. a T cell proliferation was measured after incubation for 72 h in 96-well round-bottomed plates. b at a DC:T ratio of 1:2, the percentages of CD4⁺IFN-γ⁺ T cells (Th1), CD4⁺IL-4⁺ T cells (Th2), and CD4⁺CD25⁺Foxp3⁺ T cells (Tregs) among CD4⁺CD25⁻ T cells were quantified after incubation for 6 days. c CD4⁺CD25⁻, CD8⁺T cells, and DCs were isolated from s.c. transplanted hepatoma tissue from WT BALB/c mice in the presence or absence of treatment with anti-FGL2. The percentages of Th1 and Th2 cells and Tregs among CD4⁺CD25⁻ T cells were measured as described in (b). Data represent the percentage of positive cells (mean ± SEM). *P < 0.05, **P < 0.01 (Mann–Whitney U test) as compared with the negative control group