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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Soluble fibrinogen-like protein 2 promotes the growth of hepatocellular carcinoma via attenuating dendritic cell-mediated cytotoxic T cell activity

Fig. 3

Fgl2 knockout promotes DC-mediated T cell proliferation in s.c. transplanted hepatomas. CD4+CD25 and CD8+ T cells and DCs were isolated from s.c. transplanted hepatoma tissue from Fgl2−/− or WT BALB/c mice using magnetic beads. T cells (1 × 105) were dyed with CFSE (5 μM) and mixed with DCs at different ratios (DC:T cells, 1:2, 1:5, 1:10, and 1:50) in the presence of 200 IU/mL murine IL-2 in culture. a T cell proliferation was measured after incubation for 72 h in 96-well round-bottomed plates. b at a DC:T ratio of 1:2, the percentages of CD4+IFN-γ+ T cells (Th1), CD4+IL-4+ T cells (Th2), and CD4+CD25+Foxp3+ T cells (Tregs) among CD4+CD25 T cells were quantified after incubation for 6 days. c CD4+CD25, CD8+T cells, and DCs were isolated from s.c. transplanted hepatoma tissue from WT BALB/c mice in the presence or absence of treatment with anti-FGL2. The percentages of Th1 and Th2 cells and Tregs among CD4+CD25 T cells were measured as described in (b). Data represent the percentage of positive cells (mean ± SEM). *P < 0.05, **P < 0.01 (Mann–Whitney U test) as compared with the negative control group

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