Fig. 6

sFGL2 inhibits Akt and p38 phosphorylation and signaling in DCs. Bone-marrow cells were isolated from BALB/c mice and stimulated with 10 ng/mL IL-4 and 10 ng/mL GM-CSF for 6 days in order to form immature BMDCs prior to a incubation with different concentrations (0, 0.5, 1.0, and 2.0 μg/mL) of recombinant murine FGL2 for 16 h, followed by 0.5 μg/mL LPS stimulation for 30 min. Proteins were detected with anti-mTOR, anti-Akt, anti-NF-κB-p65, anti-CREB, anti-p38, anti-ERK1/2, and their respective phosphorylated forms of the antibodies. b A representative result of three independent experiments and c the IOD ratio detected protein to that of the internal control are presented as the mean ± SEM. *P < 0.05, **P < 0.01 (Mann–Whitney U test) as compared with each other