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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Long non-coding RNA GAS5 inhibits DDP-resistance and tumor progression of epithelial ovarian cancer via GAS5-E2F4-PARP1-MAPK axis

Fig. 2

GAS5 over-expression caused cell cycle arrest and promoted apoptosis of OC cells. a LV5-GAS5 or the empty LV5 vector plasmids were transfected into OC cells. 72 h later, overexpression efficient of GAS5 was evaluated by RT-qPCR. b 72 h after transfection of LV5-GAS5 or the empty vector, flow cytometry assay were performed to detect the cell cycle impacted by overexpression of GAS5 in OC cell lines SKOV3 and HEY, and cell cycle-related protein levels were then assessed by Western-blot assay. β-actin was served as the internal control. c After transfection, cells were harvested and stained by Annexin V-PE and 7-AAD, and apoptosis rates were analyzed by flow cytometry, and apoptosis-related protein levels were then assessed by Western-blot assay. β-actin was served as the internal control. d Transwell assay were performed to detect the migration rate of SKOV3 and HEY. *P < 0.05 versus control groups

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