Fig. 4

GAS5 regulated the expression of PARP1 and effected MAPK pathway in OC cells. a The protein level of PARP1 were assessed by Western-blot assay. GAPDH was served as the internal control. b The mRNA level of PARP1 were assessed by RT-qPCR assay. c PARP1 transcriptional activities of OC cells stably transfected with LV5-GAS5 or the empty vector were assessed by luciferase reporter assay. d The protein level of MAPK pathway members were assessed by Western-blot assay. GAPDH was served as the internal control. e GO analysis showed the top 30 pathway enrichment affected by GAS5 over-expression. *P < 0.05 versus control groups