Fig. 6

The expression of GAS5 in cytoplasm could be elevated by Rapamycin. a The mRNA and protein level of GAS5 were assessed by RT-qPCR in OC cell lines treated by Rapamycin. b Cy3-labeled GAS5 and DAPI-labeled U6 probes were obtained from RiboBio. RNA FISH were performed using fluorescent in situ hybridization kit (RiboBio) according to manufacturer’s instructions. c Flow cytometry assay were performed to detect the cell cycle impacted by Rapamycin treating in OC cell lines SKOV3 and HEY. *P < 0.05 versus control groups