Fig. 2

RUNX1 affects the progression and metastasis of colorectal cancer in vitro and vivo. a Stable RUNX1-expression and silencing cell lines were established in HCT116, RKO and SW480 cells. Expression of RUNX1 mRNA and protein detected by qPCR and western blot analysis in HCT116, RKO and SW480 cells with RUNX1 overexpressed and silencing. b Migration and invasion ability detected by Transwell assays in HCT116, RKO and SW480 cells with RUNX1 overexpressed and silencing. c Migration and invasion ability detected by Wound healing in HCT116, RKO and SW480 cells with RUNX1 overexpressed or silencing. d General form of splenic metastasis and HE staining in HCT116/Vector and HCT116/RUNX1 group. Metastatic tumor number of splenic metastasis was also compared