Fig. 3

RUNX1 promotes colorectal cancer progression and metastasis via EMT. a Relationship of actin cytoskeleton and focal adhesion regulation with RUNX1 expression was shown using TCGA (n = 465) and GSE17538 (n = 177). b Enrichment analysis of GO-Cellular components showed cytoskeleton、cell junctions、actin cytoskeleton and cell-cell junction were enriched with RUNX1 expression. (Database source: Gene Transcription Regulation Database: http://gtrd.biouml.org/ and DAVID: http://david.ncifcrf.gov/). c Cytoskeleton detected by FITC-phalloidine staining in HCT116, RKO and SW480 cells with RUNX1 overexpressed and silencing. d Expression of EMT-related molecules detected by western blot in HCT116/vector, HCT116/RUNX1, SW480/scramble and SW480/shRUNX1 groups. e Immunofluorescene assay of E-cadherin, N-cadherin and vimentin in HCT116/vector, HCT116/RUNX1, SW480/scramble and SW480/shRUNX1 cells. Relevant immunofluorescence scores were shown. f E-cadherin、N-cadherin and Vimentin expression detected by IHC in HCT116/Vector and HCT/RUNX1 group. Pathological scores of each were obtained