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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer

Fig. 4

RUNX1 performing functions in CRC development via Wnt/β-catenin signaling pathway. a Enrichment of WNT signaling pathway with RUNX1 expression was shown using enrichment analysis of TCGA (n = 465) and GSE17538(n = 177). b RUNX1 expression correlates to CTNNB1 gene (β-catenin coding gene) positively, given by a colorectal cancer data set of GSE17538. c RUNX1 relates to CTNNB1 gene positively in different CRC cell lines. d Expression of RUNX1 and β-catenin protein in HCT116/Vector, HCT116/RUNX1, SW480/scramble and SW480/shRUNX1 groups. e Expression of nuclear or cytoplasmic RUNX1 and β-catenin protein in HCT116 and SW480 cells with RUNX1 overexpressed and silencing. The control for normalization of nuclear and cytoplasmic separation was PCNA and GAPDH, respectively. f Co-immunoprecipitation assay observed RUNX1 interacts with β-catenin. g Immunofluorescence assay of β-catenin shows higher level of nuclear β-catenin in the RUNX1-overexpressing groups. h Expression of RUNX1 protein and targeted genes activated by Wnt signaling pathway in HCT116/Vector, HCT116/RUNX1, SW480/scramble and SW480/shRUNX1 groups

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