Fig. 4

KIFC1 induces EMT in HCC cells. a Cell morphology changed from a spindle-like appearance to a cobblestone-like appearance in KIFC1-knockdown 7701 cells. b There were more pseudopodia formation in HepG2-KIFC1 cells compared with HepG2-vector cells. The bar indicated 40 μm (magnification: 600 ×). c Pseudopodia were formed in 7701-KIFC1-control cells and decreased in KIFC1-knockdown cells. The bar indicated 40 μm (magnification: 600 ×).d The colocalization of F-actin and Tks5 in KIFC1 knockout (KO) cells and KIFC1 overexpression (OE) cells. The white arrows indicated the colocalization sites. The bar indicated 40 μm (magnification: 600 ×). e In KIFC1-overexpressing cells, mesenchymal markers such as vimentin and Twist1 were increased, and the epithelial marker E-cadherin was downregulated