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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: KIFC1 is activated by TCF-4 and promotes hepatocellular carcinoma pathogenesis by regulating HMGA1 transcriptional activity

Fig. 7

KIFC1 is activated by the Wnt/β-catenin pathway. a Western blot analysis of the expression of KIFC1 and Wnt/β-catenin pathway components in 7402 cells treated with Wnt3α medium or LiCl (30 mM) for the indicated times. b and c Western blot analysis revealed the expression of KIFC1 and Wnt/β-catenin pathway components with different doses of inhibitors. XAV939 inhibits tankyrase, and tankyrase is an enzyme that mediates the poly-ADP-ribosylation of Axin. Axin subsequently undergoes degradation. ICRT3 combines with β-catenin and inhibits its combination with TCF-4. Both inhibitors inhibit Wnt/β-catenin pathway activity. d A dual-luciferase assay showed the transcriptional activities regulated by iCRT3 treatment in the indicated KIFC1 wild type and mutant promoters. Data are presented as the mean ± SD, * P < 0.01, ** P < 0.001. e A dual-luciferase assay revealed the different transcriptional activities regulated by LiCl treatment in the indicated KIFC1 truncation, wild type and mutant promoters. The KIFC1 promoter sequences were mutated from 5′-GCTTTGAATC-3′ to 5′- TAAAAATCGT − 3′. Data are presented as the mean ± SD, * P < 0.01, ** P < 0.001. f Western blot analysis revealed the expression of KIFC1 and other TCF-4 regulating genes in TCF-4-silenced and TCF-4 overexpression 7402 and HepG2 cells. g Illustration of predicted binding sites from the ALGGEN-PROMO database. h ChIP assay was performed with TCF-4 antibody (2565, Cell signaling technology) IgG was used as the negative control. Quantitative PCR was conducted at the promoter regions of KIFC1 with HepG2 and Huh7 cell lines. i The correlation between KIFC1 and Cyclin D1 was performed in 40 HCC samples with qRT-PCR. j KIFC1 and Cyclin D1 expression in the same HCC sample was tested with IHC assay

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