Fig. 1

Design and expression of the GrB-Fc-4D5 construct. a cDNA of GrB, IgG-Fc region and an anti-HER2 scFv were fused together with a flexible GGGGS linker by overlapping PCR. The resulting plasmid was then cloned into the mammalian expression pSECTag-A vector for either transient HEK-293E or stable CHO expression of the fusion protein. b SDS-PAGE of GrB-Fc-4D5 and GrB(S195A)-Fc-4D5 confirmed > 95% purity of the fusion proteins and that both constructs are monomers under reducing conditions and homodimers under non-reducing conditions