Fig. 4

Mechanistic studies of GrB-Fc-4D5. a Western blot analysis of MEF3.5^−/− (antigen negative) and MDA-MB-231 (targeted) cell extracts for pAkt, Caspase 9 and cleaved PARP after treatment with 20 nmol/L of GrB-Fc-4D5 for 0, 24 and 48 h. b Western blot analysis of cell extract for pHer2 (Y877) was assayed against SKBR3 after treatment with 100 nmol/L of either Herceptin or GrB-Fc-4D5 for 0, 24 and 48 h. β-actin was used as a control for protein loading