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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: TET1-mediated DNA hydroxymethylation activates inhibitors of the Wnt/β-catenin signaling pathway to suppress EMT in pancreatic tumor cells

Fig. 5

TET1-dependent demethylation induces transcriptional activation of SFRP2. a QRT-PCR of mRNA levels of DKKs and SFRPs in BX-TET1-OE and BX-TET1-NC cells. b Efficiency of si-SFRP2 was detected by qRT-PCR. c Transwell assays revealed that SFRP2 knockdown diminished TET1 inhibition of cell migration and invasion, **P < 0.01. d Western blot analysis of total β-catenin distribution after SFRP2 knockdown in BX-TET1-OE and BX-TET1-NC cells. e ChIP analysis of TET1 binding to the SFRP2 promoter. f 5-hmC and 5-mC content analysis in the SFRP2 promoter; MspI digestion indicated the 5-hmC level, HpaII digestion indicated 5-hmC and 5-mC levels, and the control indicated the total amount of 5-C, **P < 0.01. g BSP indicated the changes in methylation levels in the SFRP2 promoter

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