Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: AHR and GPER mediate the stimulatory effects induced by 3-methylcholanthrene in breast cancer cells and cancer-associated fibroblasts (CAFs)

Fig. 5

AHR and GPER are involved in the up-regulation of cyclin D1 by 3MC. a Cyclin D1 mRNA expression in SkBr3 cells and CAFs treated with vehicle (−) and 1 μM 3MC, as indicated. mRNA expression of cyclin D1 in SkBr3 cells (b) and CAFs (d) upon treatments for 18 h with vehicle (−) and 1 μM 3MC alone and in combination with either 1 μM AHR inhibitor CH223191 or 100 nM GPER antagonist G15, as indicated. Data obtained by real-time PCR in three independent experiments performed each in triplicate were normalized to 18S expression and shown as fold changes of Cyclin D1 expression induced by treatments with respect to cells treated with vehicle (−). Cyclin D1 protein levels in SkBr3 cells (c) and CAFs (e) upon treatments for 18 h with vehicle (−) and 1 μM 3MC alone and in combination with either 1 μM AHR inhibitor CH223191 or 100 nM GPER antagonist G15, as indicated. Cyclin D1 protein levels in SkBr3 cells (f) and CAFs (j) upon treatments for 18 h with vehicle (−) and 1 μM 3MC alone or in combination with 5 μM CYP1B1 activity inhibitor TMS. Cyclin D1 protein levels in SkBr3 cells (g) and CAFs (k) transiently transfected with shRNA or shCYP1B1 for 24 h and then treated for 18 h with vehicle (−) and 1 μM 3MC. h, l Efficacy of CYP1B1 silencing. Cyclin D1 protein levels in SkBr3 cells (i) and CAFs (m) treated for 18 h with vehicle (−) and 1 μM 3MC alone or in combination with 100 nM SP1 inhibitor Mithramycin A (MTM A). β-actin serves as a loading control. Results shown are representative of three independent experiments. (■) P < 0.05 for cells receiving treatments versus vehicle (−)

Back to article page