Fig. 3

Global changes in gene expression in BxPC-3 cells transfected with ANLN siRNA. a, The heat map of the differentially expressed genes with a fold change of greater than 2.5 or less than − 2.5 in ANLN RNAi relative to NC. b, A volcano plot showing the differentially expressed genes with a fold change of greater than 2.5 or less than − 2.5 in ANLN RNAi relative to NC. c, The top twenty-five regulated Gene ontology (GO) biological process terms. d, The heat map of 54 cell-cell adhesion-related genes. e, The selected candidate genes from microarray experiments were confirmed by qRT-PCR after ANLN RNAi transfection in BxPC-3 cells. **P < 0.01 compared with the NC group