Fig. 1

TFEB is induced upon TGF-β treatment via the canonical smad pathway in Smad4-positive PC cells. a Analysis of expression correlation based on a TCGA dataset of 184 pancreatic cancer patients with or without smad4 gene mutation or deletion. b Western blot revealing TFEB protein expression in MIA PaCa-2 and PANC-1 cells by treatment with TGF-β (10 ng/mL) for the times indicated. c Cell fractionation was performed to separate nuclear and cytoplasmic protein fraction from without or with TGF-β (10 ng/mL) for 48 h in MIA PaCa-2 and PANC-1 cells. d Western blot showing phosphorylated Smad2 (p-Smad2) expression in MIA PaCa-2 and PANC-1 cells by treatment with TGF-β (10 ng/mL) for the times indicated. e Western blot revealing TFEB expression in MIA PaCa-2 and PANC-1 cells treated with TGF-β (10 ng/mL) and/or LY364947 (10 μmol/L) for 48 h. f Immunofluorescence of PANC-1 cells after treated with TGF-β (10 ng/mL) and/or LY364947 (10 μmol/L) for 48 h