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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells

Fig. 3

TFEB-driven autophagy is required for TGF-β-induced migration and metastasis of PC cells. a Transwell assay showing migrated MIA PaCa-2 and PANC-1 cells pre-transfected with shTFEB or/and CQ followed by a 48-h treatment with TGF-β (10 ng/mL). Data are presented as the means ± SD from 3 independent experiments (***, p < 0.001). b Transwell assay showing migrated MIA PaCa-2 and PANC-1 cells transfected with shTFEB or/and shATG5 followed by a 48-h treatment with TGF-β (10 ng/mL). Data are presented as the means ± SD from 3 independent experiments (***, p < 0.001). c Wound healing assays were used to examine cell migration in PANC-1 cells pre-transfected with shTFEB or/and CQ followed by a 48-h treatment with TGF-β (10 ng/mL); the right panel is the quantification of the wound-healing percentages. d Wound healing assays were used to examine cell migration in PANC-1 cells transfected with shTFEB or/and shATG5 followed by a 48-h treatment with TGF-β (10 ng/mL); the right panel is the quantification of the wound-healing percentages. e Liver metastasis model by nude BALB/c mice, the left panel is representative image, statistical analysis of the average numbers of visible liver is in right panel. Data are presented as mean ± SD (n = 6). Scale bar: 5 mm. ***, p < 0.001. f The body weight of the mice in each group was measured once a week from the time of cells injection and Kaplan-Meier survival curves of each indicated group (n = 6). n.s., no significance; *, p < 0.05; **, p < 0.01

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