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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells

Fig. 4

TGF-β facilitates endocytosis of integrin and focal adhesion disassembly mediated by TFEB. a Western blot revealing expression of indicated proteins in MIA PaCa-2 and PANC-1 cells after treatment with TGF-β (10 ng/mL, 48 h). b Itgα5β1 levels in MIA PaCa-2 and PANC-1 cells treated with CHX (25μg/mL) over the indicated periods after pre-transfected with shTFEB followed by a 48-h treatment with TGF-β (10 ng/mL). c Biotinylation-based internalization and recycling assays showing internalized and recycled Itgα5 in PANC-1 cells after pre-transfected with shTFEB followed by a 48-h treatment with TGF-β (10 ng/mL). d Western blot of focal adhesion disassembly assay via assessing p-FAK (Tyr397) expression after nocodazole washout (U means untreated). e Confocal images of paxillin and zyxin in PANC-1 cells pre-transfected with shTFEB followed by a 48-h treatment with TGF-β (10 ng/mL)

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