Fig. 5

RAB5A is TFEB direct target for TGF-β induced endocytosis of integrin. a PANC-1 cells were co-transfected with pRL-TK and CLEAR site-pGL3 and treated with TGF-β (10 ng/mL, 48 h) and/or pre-transfected with shTFEB. Luciferase activity was normalized to renilla luciferase activity and expressed as the mean ± SD. *, p < 0.05; **, p < 0.01. b Real-time PCR showing relative mRNA level of RAB5A, LAMP-1 and CTSD in PANC-1 cells treated with TGF-β (10 ng/mL, 48 h) and/or pre-transfected with shTFEB. c Western blot analysis of RAB5A, LAMP-1 and CTSD in MIA PaCa-2 and PANC-1 cells by treatment with TGF-β (10 ng/mL) for the times indicated. d Western blot revealing relative mRNA level of RAB5A, LAMP-1 and CTSD in PANC-1 cells treated with TGF-β (10 ng/mL, 48 h) and/or pre-transfected with shTFEB. e Confocal images of RAB5A and Itgα5 in PANC-1 cells pre-transfected with shTFEB followed by a 48-h treatment with TGF-β (10 ng/mL)