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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: CRABP2 regulates invasion and metastasis of breast cancer through hippo pathway dependent on ER status

Fig. 2

Knockdown of CRABP2 promotes EMT, metastasis and invasion of ER+ breast cancer cells in vitro and in vivo. (a) Wound-healing, (b) migration and (c) invasion experiments were conducted in wild-type cells (sh-NC), T47D and MCF7 cells with stable knockdown of CRABP2 (sh-CRABP2–1, sh-CRABP2–2). The percent of wound closure and migratory and invasive cells numbers were counted. P-values were calculated by the Student’s t-test. d-e Analysis of E-cadherin, ZO-1, and Vimentin expression in T47D and MCF7 cells expressing CRABP2 shRNA (sh-CRABP2–1, sh-CRABP2–2) or CRABP2 siRNA (si-CRABP2)and shRNA (sh-NC) or siRNA(si-NC) by Western blotting (d) and immunofluorescence (e). Scalebar, 10 μm. f Representative images, HE staining and relative number of metastatic lung nodules of lung tissues of mice. (n = 6 per group). g Immunohistochemical staining of CRABP2, E-cadherin, Vimentin in sh-NC group and sh-CRABP2 group. Scalebar, 145 μm. Data are the mean ± S.D. of three independent experiments performed in triplicate. *, P < 0.05; **, P < 0.01; ***, P < 0.001

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