Fig. 6

CRABP2 interacts Lats1 and regulates the degradation of Lats1 in breast cancer cells. a-b Subcellular localization of CRABP2 and Lats1 in T47D (a) and BT549 (b) cells by immunofluorescence. Scalebar, 10 μm. c In the left, extracts of T47D cell were coimmunoprecipitated (IP) with anti-Flag antibody or IgG and analyzed by anti-CRABP2 antibody with Western blotting. In the right, extracts of T47D cell were coimmunoprecipitated (IP) with anti-CRABP2 antibody or IgG and analyzed by anti-Lats1 antibody with Western blotting. d Detection in BT549 cells by (c) method. e-f Cycloheximide (100 μg/ml) contact T47D (e) and BT549 (f) cells with gradient time. The protein expression was examined by Western blotting. Quantification of Lats1 protein levels was determined using Image J software normalized to GAPDH (bottom panel). g-h MG132 (5 μM) contact T47D (g) and BT549 (h) cells with 24 h. The protein expression was examined by Western blotting. Data are the mean ± S.D. of three independent experiments performed in triplicate