Fig. 2

AMPKα plays a central role in cell cycle arrest induced by metformin and promotes Yap1 degradation in the BLCA cells. a. Western Blot was used to detect the expressions of AMPK and Yap1 in T24 and Sw780 cells treated with metformin at different concentrations (5 mM, 10 mM and 20 mM). b. The cell viabilities of T24 and Sw780 cells were determined when the cells were treated by metformin with/without AMPK inhibitor Compound C (CC) at the time point of 48 h. c. The cell cycle analysis was performed using Flow Cytometry when T24 and Sw780 cells were co-treated by metformin with/without CC for 48 h. d. The expressions of AMPKα, CCND1, CCNNE1/2 CDK4/6, CDK2 and Yap1 were detected by Western Blot in the T24 and Sw780 cells co-treated by metformin with/without CC for 48 h. e. Immunofluorescence was used to examine the overall distribution of Yap1 in the T24 and Sw780 cells challenged by metformin of different concentrations. * means P < 0.05 and ** means P < 0.01 compared to the control group