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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Metformin targets a YAP1-TEAD4 complex via AMPKα to regulate CCNE1/2 in bladder cancer cells

Fig. 3

Yap1 knockdown induces the cell cycle arrest at G1 phase in T24 and Sw780 cells. a. Yap1-specific small interfering (si) RNA was used to down-regulate the expression of Yap1 and Western Blot method was used to detect the expressions of Yap1 in the T24 and Sw780 cells. b. Yap 5SA mutant plasmid (Mutation of all five serine to alanine at S61A, S109A, S127A, S164A, S381A) was transfected into T24 and Sw780 cells to generate a Yap1 mutant resistant to phosphorylation by its up-stream kinases. c. the cell viabilities of T24 and Sw780 cells were determined using MTS assay when transfected with Yap1 specific siRNA or Yap 5SA plasmid. d. The cell cycle was determined with Flow Cytometry when T24 and Sw780 cells were interfered by Yap1-siRNAs or Yap 5SA plasmid. e. The protein expressions of CCNE1/2, CCND1, CDK2 and CDK4/6 were detected in the T24 and Sw780 cells interfered by Yap1-siRNAs or combined transfection with Yap 5SA plasmid. ** means P < 0.01 and *** stands for P < 0.005, compared to the control group

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