Fig. 5
From: Metformin targets a YAP1-TEAD4 complex via AMPKα to regulate CCNE1/2 in bladder cancer cells
Yap1 regulates CCNE1 and CCNE2 gene expressions via TEAD4. a The relative mRNA expressions of CCNE1 and CCNE2 in T24 and Sw780 cells interfered with Yap1-siRNAs. b The relative mRNA expressions of CCNE1 and CCNE2 in T24 and Sw780 cells interfered with TEAD4-siRNAs. c Consensus binding motif of TEAD4 and the binding sites of TEAD4 on the promotors of CCNE1 and CCNE2 were predicted using JASPAR database. d ChIP-qPCR analysis was used to confirm the enrichment of CCNE1 and CCNE2 at the different binding sites predicted using Yap1antibody and a negative (IgG) antibody in T24 and 5637 cells. e Relative luciferase activities were determined in cell lysates for quantitation of CCNE1 and CCNE2gene expressions in T24 and Sw780 cells