Fig. 6
From: Metformin targets a YAP1-TEAD4 complex via AMPKα to regulate CCNE1/2 in bladder cancer cells
metformin inhibits the proliferation of BLCA cells in vivo. a. The total body weight of nude mice was measured when the treatment with metformin at the dosages of 150 mg/kg/d and 250 mg/kg/d was given. b. Tumor growth in xenograft mice receiving metformin treatment was recorded. c. The protein expressions of AMPK, Yap1 CCNE1 and CCNE2 was determined with Western Blot in the tumor tissues from the xenograft mice. d. The expressions of PCNA, Yap1, CCNE1 and CCNE2 were determined by Immunohistochemistry (IHC) staining derived from the xenograft. PCNA, Yap1, CCNE1 and CCNE2 positive cells were counted in 12 fields in each group. e. The percentage of PCNA, Yap1, CCNE1 and CCNE2 positive cells was calculated and presented as mean ± SD. All IHC analysis was performed with Image J software. * means P < 0.05 and ** means P < 0.01, compared to the control group