Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: The interaction of YBX1 with G3BP1 promotes renal cell carcinoma cell metastasis via YBX1/G3BP1-SPP1- NF-κB signaling axis

Fig. 6

The interaction between YBX1 and G3BP1 in nude mice. (a). ACHN-Scr cells and ACHN-shG3BP1 cells were labeled with luciferase gene, and 1 × 106 cells were injected into the sub-renal capsule. Tumor growth was monitored by IVIS imaging system, and representative bioluminescence images were shown. Liver (b) and lung (e) metastases were examined by IVIS bioluminescence imaging system, and representative images were shown. Representative H&E staining of Liver (c) and lung (f) isolated from orthotopic xenograft models to detect metastatic foci. Tumor metastatic foci in liver (d) and lung (g) were quantified and compared using five random views under the microscope. (h) Co-localization of YBX1 and G3BP1 was examined by Immunofluorescence in xenograft model. (i) Representative tumor sections from G3BP1 knockdown and control mice were subjected to immunohistochemistry staining using the YBX1, G3BP1 and SPP1 antibodies. Scale bar = 50 μm. Statistically significant differences were indicated: **, P < 0.01; ***, P < 0.001

Back to article page