Fig. 4

Effect of sorafenib and HGFK1 on cell apoptosis of RCC. a Ketr-3 and ACHN cells were treated with sorafenib and/or rHGFK1 for 48 h and stained with an Annexin V-FITC/PI apoptosis kit, and then analyzed with a flow cytometer. b Quantification of cell apoptosis is shown for Ketr-3 and ACHN induced by sorafenib and/or rHGFK1. c Western-Blotting was used to detect the cleavage of PARP induced by sorafenib and/or rHGFK1 in Ketr-3 and ACHN cells. All data shown represent the mean ± SD from at least three independent experiments. Significant differences are denoted by *** for p < 0.001