Fig. 5

HDAC6 – RUNX2 complex controls a specific subset of target genes. TPC1 cells were transfected with siRNA against HDAC6 or RUNX2, after 48 h RNA-Seq analysis was performed. Venn diagram (a) and GO analysis (b) were used to analyze results. Common up- and down- regulated genes were validated on an independent set of RNAs (c-f). Histograms represent the relative fold change +/− SD of silenced cells as compared to control cells. Each experiment represents the average of three independent replicates. * p < 0.05. TPC1 were also assessed for the binding of RUNX2 (g) and HDAC6 (h) to the promoters of 3 of the genes regulated by the HDAC6-RUNX2 complex. Histograms represent the average enrichment of the indicated genomic regions in the immunoprecipitated DNA expressed as percentage of the Input. Data are expressed as mean values +/− SEM of a technical triplicate and are representative of at least two independent experiments. * p < 0.05