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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Cardamonin inhibits breast cancer growth by repressing HIF-1α-dependent metabolic reprogramming

Fig. 6

The mTOR/p70S6K pathway mediates the inhibitory effect of cardamonin on HIF-1α and cell viability. a Cardamonin (20 μM) treatment for 1, 6, 12 and 24 h reduced the phosphoryaltion of PI3K, AKT, mTOR and P70S6K in MDA-MB-231 cells. b Cardamonin (5, 10, 20 and 40 μM) treatment for 24 h decreased the phosphorylation of PI3K, AKT, mTOR and P70S6K in MDA-MB-231 cells. c Cardamonin (20 μM), as early after treatment for 0.5 h, mitigated the phosphorylation of mTOR and P70S6K in MDA-MB-231 cells. d, e Pretreatment with MHY1485 (mTOR activitor, 14 μM) for 30 min reversed the inhibitory effect of cardamonin (20 μM) on the protein expression of p-mTOR, HIF-1α and PDHK1. f CCK-8 analysis showed that pretreatment with MHY1485 rescued cell viability in cardamonin-treated MDA-MB-231 cells. Data are shown as mean ± SD; *, P < 0.05, **, P < 0.01; ***, P < 0.001. n ≥ 4

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