Fig. 1

Dropwort extract affects viability and impairs migration and invasion of MPM cells. a Viability of MPM cell lines (MSTO-211H, MPP89, NCI-H28 and NCI-2052) and normal untransformed mesothelial cells (HMC) treated for 72 h with different doses of Fil.v. extract (0-200 μg/ml). b IC-50 value calculated by Compusyn software. Data are represented as mean +/− SD. Statistics (t-test): p < 0.05. c Colony forming assay. MSTO-211H, MPP89, NCI-H28 and NCI-2052 cells were treated with different doses of Fil.v. extract (0-200 μg/ml). Histograms showing average colony counts from triplicate experiments. Bars indicate the average of three independent experiments. Statistics (t-test): p < 0.05. d, f Left side: representative micrographs of wound healing closure assays from MSTO-211H (d) and MPP-89 (f) cells treated for 36 h with 12.5 μg/ml of Fil.v. extract. Right side: histogram showing the healing closure efficiency of the cells treated with vehicle or the Fil.v. extract (6 μg/ml and 12 μg/ml) after different times of treatment (0, 24 and 36 h). Bars indicate the average of three independent experiments. Statistics (t-test): p < 0.05. e, g Percentage of invading cells over vehicle. MSTO-211H cells (e) or MPP-89 cells (g) were treated at the indicated doses of extract. Error bars represent mean +/− SD. Statistics (t-test): p < 0.05