Fig. 7

Depletion of YAP and/or TAZ affects the response of MPM cells to Dropwort extract. a Histograms showing the average percentage of viability of MSTO-211H cells transfected with a siRNA control or with a siRNA against YAP, or TAZ, or TEAD and treated with different doses of Fil.v. extract for 72 h as indicated. Statistics (t-test): p < 0.05. b Graphs indicating the fitness (impedance) of MSTO-211H cells transfected as from (a) and subsequently treated with Fil.v. extract (50 μg/ml) for 0–2000 min. Statistics (t-test): p < 0.05. c Quantitative-PCR. Histograms showing the relative level of the indicated genes in MSTO-211H cells treated as from (a). Bars indicate the average of three independent experiments. Statistics (t-test): p < 0.05. d Histograms showing the average percentage of viability of MSTO-211H cells transfected with a siRNA control or concurrently with siRNA against YAP and TAZ and treated with different doses of Fil.v. extract for 72 h as indicated. Statistics (t-test): p < 0.05. e Metabolic response of MSTO-211H cell lines differently depleted for siRNA control or YAP or TAZ and treated with 50 μg/ml of Fil.v. extract for 24 h. PCA models built on the ¹H-NMR dataset of media samples. f Histogram show the average percentage of glucose levels obtained from analysis of the variable importance in projection (VIP) of MSTO-211H cells depleted and treated as from e