Fig. 3
3D185 inhibited the survival and polarization of M2-like macrophages induced by CSF-1/CSF-1R. a The effect of 3D185 and PLX3397 on the survival of CSF-1-induced murine and human macrophages. Murine bone marrow and human monocytes were stimulated with CSF-1 for 7 days together with the indicated inhibitor, and cell viability was evaluated by the CCK-8 assay. The IC50 values are shown as the mean ± SD (nM) from two independent tests. B-E, To test the impact of 3D185 on the polarization of CSF-1-induced macrophages, murine bone marrow cells and human monocytes were induced to mature into macrophages with CSF-1 for 7 days and then stimulated with CSF-1, IL-4 and IL-13 for an additional 48 h. The expression of the markers MHC-II (b), CD86 (c), and CD206 (d) on induced murine macrophages and CD206 expression on induced human macrophages (e) were determined by flow cytometry, and the mean fluorescence intensity (MFI) was analyzed with FlowJo. Representative data from two independent experiments. f Murine bone marrow cells (left panel) and human monocytes (right panel) were induced to mature into macrophages with CSF-1 for 7 days and then stimulated with CSF-1, IL-4 and IL-13 and treated with vehicle or the indicated inhibitor for an additional 48 h. Then, all cells were collected to count viable cells by staining with trypan blue