Fig. 2

WD40 domains of DTL played important roles in interaction with PDCD4. a Schematic diagram of DTL primary structure and truncated plasmid construction. b Co-immunoprecipitation assay was performed using truncated DTL fragments. Flag-DTLs were transfected into 293 T cells using Lipofectamine 2000 Transfection reagent. Immunoprecipitation was performed by PDCD4 antibody and immunoblotting by Flag antibody. c Three-dimensional structure of DTL was predicted by I-TASSER using ab initio protein structure prediction technique. DTL contained two β propellers, one of them was formed by WD40 domain located in the N-terminal, as shown in red. d The interaction between DTL and PDCD4 was shown. PDCD4 was indicated in red, and WD40 domain of DTL in green. The results showed the WD40 domains of DTL interacted with PDCD4